Congratulations to Chava for successfully defending his doctoral dissertation entitled: “Monitoring the spread of plasmid-mediated antibiotic resistance in agricultural soil.”
The first two chapters from his work are available online! Chapter 1: Plasmids, a molecular cornerstone of antimicrobial resistance in the one health era. In this review, we outline the global significance of antimicrobial resistance with a focus on plasmids as key players in the dissemination of antibiotic resistance genes. Utilizing a One Health approach, we explore the interconnectedness of habitats and discuss factors that influence the ecology and evolution of plasmids.
Chapter 2: Detection of rare plasmid hosts using a targeted Hi-C approach. We first established the detection limit of Hi-C, a proximity ligation method, for associating a plasmid with its host in soil. For a plasmid to be associated with its host, the pair needed to be present at, or above, a relative abundance of .001%. Then, we combined Hi-C with a target capture approach aimed at enriching for DNA of the focal plasmid. This enrichment approach, referred to as Hi-C+, increased the detection limit of Hi-C 100-fold.